The Biosensis NGFR/p75ECD Rapid™ enzyme-linked immunosorbent assay (ELISA) kit is a sandwich ELISA that allows the quantification of the extracellular domain (ECD) of human p75 neurotrophin receptor in less than four hours in urine only if used as directed.
- Complete kit with precise instructions
This optimised human p75ECD ELISA assay demonstrates an increased sensitivity, lowering the LOD about 8-fold to the previous assay version. It represents the most sensitive NGFR/p75ECD ELISA assay available today, with a detection limit of at least 4 pg/ml, and a quantification range of 7,8 to 500 pg/ml.
This ELISA kit consists of a pre-coated mouse monoclonal anti-p75ECD capture antibody, a biotinylated mouse monoclonal anti-p75ECD detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of p75ECD present in samples and protein standards. A human p75ECD positive control (QC sample) is provided to assure consistent assay performance.
This NGFR/p75ECD ELISA kit employs an in-house recombinant human p75ECD-Fc chimera as standard that has been validated against an externally available p75ECD-Fc protein. While there is a current lack of a commercially available, true human p75ECD standard, this ELISA kit allows quantification of human p75ECD as p75ECD-Fc human equivalents.
The new monoclonal antibody pair yields higher OD values in urine samples, demonstrating its increased ability to detect natural p75ECD in biological samples. This means that samples assayed with BEK-2239 will give higher urine p75ECD levels, or that urine samples from healthy controls with previous urine p75ECD concentrations <LOD may read positive. Also, this kit includes a standard obtained from a different source, providing a reference standard curve different to the previous one.
Fornecimento: Each kit contains 1 or 2×96 pre-coated strip plates, protein standards, QC sample, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Atenção: For research use only. Not for diagnostic or clinical purposes.