The CheckMate™/Flexi® vector mammalian two-hybrid system provides a means to confirm, validate and study suspected interactions between two proteins or domains and can also be used to generate stable cell lines for cell-based assays.
- The Dual-Luciferase® reporter assay system is used for detection
- Interactions can be studied in the cell line of choice
- The system can allow protein expression and post-translational modifications such as glycosylation, phosphorylation and acylation
This system differs from the original CheckMate™ mammalian two-hybrid system in that the vectors are compatible with the Flexi® vector system, which allows directional cloning and rapid, efficient and high-fidelity transfer of protein coding regions between a variety of Flexi® vectors.