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Fluorometric Polymerase Activity Assay Kit, EvaEZ™

Fornecedor: Biotium
EvaEZ™
29051.EA 470 EUR
29051.
Fluorometric Polymerase Activity Assay Kit, EvaEZ™
Ensaios Ensaios enzimáticos
EvaEZ™ Fluorometric Polymerase Activity Assay Kit provides an easy and accurate way to determine the activity of a nucleic acid polymerase without using radioisotopes.

  • Just add polymerase
  • No radioisotopes needed
  • Can be used at temperatures from 4 to 75 °C

EvaEZ™ Fluorometric Polymerase Activity Assay Kit contains EvaGreen® dye together with a primed template, dNTPs, and MgCl2 in a Tris buffer system.

In the presence of DNA polymerase activity, the primer will be extended to form a double stranded product that can bind EvaGreen dye, resulting in an increase of fluorescence. The rate of increase of fluorescence is positively correlated to the activity of polymerase.

The EvaEZ™ Fluorometric Polymerase Activity Assay was developed for the measurement of Taq DNA polymerase activity but can also be used for other DNA polymerases, such as Pfu, Vent, Phusion®, Bst, Phi29, MMLV, AMV, SuperScript®, T4 DNA polymerase, T7 DNA polymerase, Klenow, and E. coli DNA polymerase I.
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