EvaEZ™ Fluorometric Polymerase Activity Assay Kit provides an easy and accurate way to determine the activity of a nucleic acid polymerase without using radioisotopes.
- Just add polymerase
- No radioisotopes needed
- Can be used at temperatures from 4 to 75 °C
EvaEZ™ Fluorometric Polymerase Activity Assay Kit contains EvaGreen® dye together with a primed template, dNTPs, and MgCl2 in a Tris buffer system.
In the presence of DNA polymerase activity, the primer will be extended to form a double stranded product that can bind EvaGreen dye, resulting in an increase of fluorescence. The rate of increase of fluorescence is positively correlated to the activity of polymerase.
The EvaEZ™ Fluorometric Polymerase Activity Assay was developed for the measurement of Taq DNA polymerase activity but can also be used for other DNA polymerases, such as Pfu, Vent, Phusion®, Bst, Phi29, MMLV, AMV, SuperScript®, T4 DNA polymerase, T7 DNA polymerase, Klenow, and E. coli DNA polymerase I.