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Amine-reactive fluorescent dyes and free acid dyes

Fornecedor: Biotium

90091.EA 234 EUR
90091.
Amine-reactive fluorescent dyes and free acid dyes
A variety of amine-reactive classic dyes for labeling primary and secondary amines on proteins and other biomolecules, as well as their non-reactive free acid and salt forms.

  • Selection of amine-reactive fluorescent dyes in a variety of colours
  • Available with succinimidyl ester (NHS ester) and isothiocyanate reactive groups
  • Variety of linker lengths for improved water solubility and reduced steric interaction between dyes and labeled molecules
  • Corresponding non-reactive dyes also available as free acids and salts

Amine-reactive fluorescent dyes are also available in a variety of colours. Succinimidyl ester (SE) compounds are the most commonly used reagents for labeling amines due to convenience of use and stability of the labeled products. SE reagents can readily react with primary and secondary aliphatic amines to form a highly stable amide bond. Labeling of water-soluble amine-containing compounds such as peptides, proteins, and oligonucleotides are typically carried out in aqueous buffer with pH of 8,0 - 9,0. Amine labeling can also be carried out in an organic solvent for compounds that have low water solubility.

The high quality (>98 % purity) single isomer fluorescein and rhodamine SE products are excellent for nucleic acid sequencing and other applications where a single species product is desired. In addition, we offer products with 6-atom aminohexanoyl spacers or long water soluble PEG linkers separating the dye and reactive group.

In addition to conjugation dyes, the amine-reactive compounds are also available for specialised applications. The amine-reactive compounds fluorescamine and CBQCA are non-fluorescent until reacted with primary amines, allowing specific detection of amines. Eosin-5-isothiocyanate is an amine-reactive photosensitiser that can be used for photoconversion of electron-rich compounds like DAB or fluorescent dyes into electron-dense materials for high-resolution electron microscopy studies. It is also a good fluorescence acceptor for fluorescein, dansyl and coumarin dyes for fluorescence resonance energy transfer (FRET).
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