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illustra™ Single Cell GenomiPhi DNA Amplification kit

Fornecedor: Cytiva
29-1080-39EA 1474 EUR
29-1080-39 29-1081-07
illustra™ Single Cell GenomiPhi DNA Amplification kit
Reagentes de ácidos nucleicos Isothermal Amplification Reagents
Single Cell GenomiPhi DNA Amplification kit combines the unique capabilities of Phi29 DNA polymerase with an optimised formulation to amplify genomic DNA from 1 to 1000 cells without background interference.

  • Whole Genome Amplification of genomic DNA from as little as a single cell
  • Novel clean-up process supresses background allowing sensitivity down to 1 fg of input DNA
  • Simple Isothermal strand displacement (MDA) technology using Phi29 DNA polymerase
  • Amplifications can be completed in less than 3 hours with less than 15 minutes hands-on time
  • Optimised to promote complete cell lysis with uniform denaturation of DNA prior to amplification

Fornecimento: Kit sufficient for 25 reactions contains Single Cell GenomiPhi Reaction buffer (1×275 µl), Single Cell GenomiPhi Enzyme mix (1×25 µl), Single Cell GenomiPhi Amplification mix (1×25 µl), Single Cell GenomiPhi DTT solution (1×100 µl), Single Cell GenomiPhi Lysis buffer (1×100 µl) and Single Cell GenomiPhi Neutralisation buffer (1×100 µl). Kit sufficient for 100 reactions contains Single Cell GenomiPhi Reaction buffer (1×1,1 mll), Single Cell GenomiPhi Enzyme mix (1×100 µl), Single Cell GenomiPhi Amplification mix (1×100 µl), Single Cell GenomiPhi DTT Solution (1×100 µl), Single Cell GenomiPhi Lysis buffer (1×200 µl) and Single Cell GenomiPhi Neutralisation buffer (1×200 µl).
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